Pipetting results can easily be influenced by user technique. To get the best results, a good pipetting technique is needed. This educational eNewsletter series will show you tips and tricks for optimal performance.
Tip 11: Tip Immersion Time
When aspirating a sample into the tip, the time of immersion in the sample is a critical factor in good pipetting practices. After releasing the plunger button smoothly, it is necessary to pause briefly before withdrawal from the sample ensuring that the full volume of sample is drawn into the tip. For larger volumes as well as viscous substances it is necessary to wait longer before withdrawing as it takes a second for the air chamber in the pipette to equalize.
More Good Pipetting tips follow with the next RAININ eNewsletter.