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Good Pipetting Practice 6 – Immersion Time and Aspiration Rate
Pipetting results can easily be influenced by user technique. To get the best results, a good pipetting technique is needed. This educational eNewsletter series will show you tips and tricks for optimal performance.
Tip 11: Tip Immersion Time
When aspirating a sample into the tip, the time of immersion in the sample is a critical factor in good pipetting practices. After releasing the plunger button smoothly, it is necessary to pause briefly before withdrawal from the sample ensuring that the full volume of sample is drawn into the tip. For larger volumes as well as viscous substances it is necessary to wait longer before withdrawing as it takes a second for the air chamber in the pipette to equalize.
When aspirating a sample into the tip, the time of immersion in the sample is a critical factor in good pipetting practices. After releasing the plunger button smoothly, it is necessary to pause briefly before withdrawal from the sample ensuring that the full volume of sample is drawn into the tip. For larger volumes as well as viscous substances it is necessary to wait longer before withdrawing as it takes a second for the air chamber in the pipette to equalize.
Tip 12: Aspiration rate
The aspiration rate of a pipette can vary and therefore can have an impact on the precision and accuracy of the results. One should always release the pipette plunger button in a smooth and controlled way. Probably the most common effect of a too quickly released plunger is that aerosols can enter the pipette shaft where it damages internal components of the pipette. This is even more dramatic in the case of liquid splash up. If this liquid is corrosive, it can cause damage to the shaft of the pipette and of course causes cross contamination of samples.
Find more information on our aerosol resistant tips with proven protection on our website.
The aspiration rate of a pipette can vary and therefore can have an impact on the precision and accuracy of the results. One should always release the pipette plunger button in a smooth and controlled way. Probably the most common effect of a too quickly released plunger is that aerosols can enter the pipette shaft where it damages internal components of the pipette. This is even more dramatic in the case of liquid splash up. If this liquid is corrosive, it can cause damage to the shaft of the pipette and of course causes cross contamination of samples.
Find more information on our aerosol resistant tips with proven protection on our website.
More Good Pipetting tips follow with the next RAININ eNewsletter.
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